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61.
Hepatitis A virus (HAV) is currently recognized as an important human food borne pathogen, and it is one of the most resistant
enteric RNA viruses, is highly infectious, and may lead to widespread outbreaks. The aim of this study was to optimize the
methods to detect HAV from artificially contaminated food. To this end, strawberry and lettuce were experimentally contaminated
with HAV suspension containing 6 × 106 copies/ml. After contamination, HAV persistence and washing procedure were evaluated at 0, 1, 3, 7, and 9 days of storage.
Five elution buffers (PBS (pH 7.4)/0.1% Tween80; 50 mM glycine/3% (wt/vol) beef extract (pH 9.5); PBS (pH 7, 4); 25 mM glycine/0.1
Tween80; and 1 M sodium bicarbonate) were used to elute the virus, and qualitative and quantitative PCR were used for HAV
detection. HAV was detected by qualitative and quantitative PCR using any of the five elution buffers, but PBS was the most
effective. Even after washing, HAV was detected up to 9 days after contamination by quantitative PCR. Quantitative PCR was
more sensitive than qualitative PCR since samples containing viral load lower than 1.4 × 103 copies/ml could not be detected by qualitative PCR. Quantitative PCR can be used for rapid detection of food borne viruses
and will help in the monitoring and control of food borne disease. 相似文献
62.
Vanessa Ecla de Oliveira Ana Paula Vanin Ani Carla Concato Wagner A. Tamagno Helen Treichel Rosilene R. Kaizer 《环境质量管理》2019,29(1):147-154
This study examined behavioral and enzymatic changes of C. elegans from its exposure to aluminum, and the resulting relationship with Alzheimer's disease. After chronic and acute exposure to aluminum, the results indicated that it alters the cholinergic status and behavior parameters of the nematode, suggesting a relationship between exposure to aluminum and the etiology of AD. 相似文献
63.
64.
Paula?Adánez SanjuánEmail author Belinda?Flem Juan?F.?Llamas Borrajo Juan?Locutura Rupérez Angel?Garcia Cortés 《Environmental geochemistry and health》2016,38(2):449-468
Changes in the principal sources of Pb in overbank sediment profiles have been documented for two Spanish areas by using Pb isotopes and Pb concentrations. These locations (Madrid and Tinto–Odiel basin) represent two of the most contaminated regions in Spain. The Community of Madrid is characterized by heavy industrial and urban activity, focused mainly in Madrid City. The Tinto–Odiel basin drains the Iberian Pyrite Belt, which hosts many polymetallic massive sulphides and is heavily affected by mining activities in their headwaters. It has been proven that the influence of anthropogenic activity is reflected in these overbank deposits by variations in Pb concentrations that, in general, correlate with shifts in the 206Pb/207Pb ratio. Rivas profile (downstream of Madrid) was found to be the most anthropogenically influenced site. The sediments within this profile which were recently deposited (170 ± 40 years BP) have the least radiogenic signatures. 206Pb/207Pb ratios ranged between 1.1763 and 1.1876 indicating significant contributions of anthropogenic Pb. In contrast, profiles upstream of Madrid possess an average 206Pb/207Pb ratio of 1.2272. It is difficult to clearly identify the most prominent source as the sediments appear to be characterized by an input from several sources. The floodplain profiles in the Tinto–Odiel basin exhibit uniform 206Pb/207Pb ratios ranging from 1.1627 (Odiel river) to 1.1665 (Tinto river). These ratios are similar to the ones possessed by sulphide ores in the area and differ from the ratios of other nonmineralized formations in the basin, indicating that mining activities are the primary, if not sole, source of Pb to the sediments. 相似文献
65.
Paula Méndez-Fernandez Graham J. Pierce Paco Bustamante Tiphaine Chouvelon Marisa Ferreira Angel F. González Alfredo López Fiona L. Read M. Begoña Santos Jérôme Spitz José V. Vingada Florence Caurant 《Marine Biology》2013,160(11):2825-2840
This study aims to assess niche segregation among the five main toothed whales that frequent the NW Iberian Peninsula waters: the common dolphin, the harbour porpoise, the bottlenose dolphin, the striped dolphin and the long-finned pilot whale. We used cadmium (Cd) and stable isotope ratios (δ13C and δ15N) as ecological tracers to assess degree of segregation in diet/trophic level and in foraging habitat, over various time-scales. δ13C values highlighted different habitats, while Cd concentrations highlighted feeding differences between oceanic and neritic species. Moreover, δ15N values suggest different trophic levels of prey targeted within oceanic and neritic species. Hence, results revealed long-term ecological segregation among five toothed whales that coexist in the NWIP and demonstrated the ability of ecological tracers to discriminate ecological niches among closely related species. 相似文献
66.
Chiral pharmaceuticals in the environment 总被引:1,自引:1,他引:0
Ana R. Ribeiro Paula M. L. Castro Maria E. Tiritan 《Environmental Chemistry Letters》2012,10(3):239-253
Many pharmaceutical pollutants are chiral, existing in the environment as a single enantiomer or as mixtures of the two enantiomers. In spite of their similar physical and chemical properties, the different spatial configurations lead the enantiomers to have different interactions with enzymes, receptors or other chiral molecules, which can give diverse biological response. Consequently, biodegradation process and ecotoxicity tend to be enantioselective. Despite numerous ongoing research regarding analysis and monitorization of pharmaceutical ingredients in the environment, the fate and effects of single enantiomers of chiral pharmaceuticals (CP) in the environment are still largely unknown. There are only few chiral analytical methods to accurately measure the enantiomeric fraction (EF) in environmental matrices and during biodegradation processes. Furthermore, the ecotoxicity studies usually consider the enantiomeric pair as unique compound. We reviewed the current knowledge about CP in the environment, as well as the chiral analytical methods to determine the EF in environmental matrices. The degradation and removal processes of CP of important therapeutic classes, usually detected in the environment, and their toxicity to aquatic organisms were also reviewed. On the other hand, this review demonstrate that despite the great importance of the stereochemistry in pharmaceutical science, pharmacology and organic chemistry, this is normally neglected in environmental studies. Therefore, CP in the environment need much more attention from the scientific community, and more research within this subject is required. 相似文献
67.
Bruno G. Lages Beatriz G. Fleury Ana M. C. Hovell Claudia M. Rezende Angelo C. Pinto Joel C. Creed 《Marine Biology》2012,159(7):1551-1559
Competition for space changes species’ distributions and community organization on tropical rocky shores, and the presence of secondary metabolites in the tissues of non-indigenous species may aid them in establishing and expanding their range through negative competitive interactions. The aim of this study was to describe the range of chemical substances produced by the non-indigenous cup corals Tubastraea coccinea and T. tagusensis and to test whether they varied in the field when the corals were placed in proximity to two local competitors. Cholest-5-en-3β-ol and 9-octadecanoic acid were two common secondary metabolites found in the tissues of Tubastraea. In the competition interaction experiment, necrosis was detected on the tissues of the coral Mussismilia hispida, and this species induced variation in sterol, alkaloid, and fatty acid production in Tubastraea tissues. In contrast, a sponge overgrew Tubastraea colonies. These results indicate that chemical defense may contribute to the ability of these non-indigenous corals to invade native communities. 相似文献
68.
Diana Paula Silva Linhares Patrícia Ventura Garcia Catarina Silva Joana Barroso Nadya Kazachkova Rui Pereira Manuela Lima Ricardo Camarinho Teresa Ferreira Armindo dos Santos Rodrigues 《Environmental geochemistry and health》2018,40(5):1713-1724
Hydrothermal areas are potentially hazardous to humans as volcanic gases such as radon (222Rn) are continuously released from soil diffuse degassing. Exposure to radon is estimated to be the second leading cause of lung cancer, but little is known about radon health-associated risks in hydrothermal regions. This cross-sectional study was designed to evaluate the DNA damage in the buccal epithelial cells of individuals chronically exposed to indoor radon in a volcanic area (Furnas volcano, Azores, Portugal) with a hydrothermal system. Buccal epithelial cells were collected from 33 individuals inhabiting the hydrothermal area (Ribeira Quente village) and from 49 individuals inhabiting a non-hydrothermal area (Ponta Delgada city). Indoor radon was measured with Ramon 2.2 detectors. Chromosome damage was measured by micronucleus cytome assay, and RAPD-PCR was used as a complementary tool to evaluate DNA damage, using three 10-mer primers (D11, F1 and F12). Indoor radon concentration correlated positively with the frequency of micronucleated cells (r s = 0.325, p = 0.003). Exposure to radon is a risk factor for the occurrence micronucleated cells in the inhabitants of the hydrothermal area (RR = 1.71; 95% CI, 1.2–2.4; p = 0.003). One RAPD-PCR primer (F12) produced differences in the banding pattern, a fact that can indicate its potential for detecting radon-induced specific genomic alterations. The observed association between chronic exposure to indoor radon and the occurrence of chromosome damage in human oral epithelial cells evidences the usefulness of biological surveillance to assess mutations involved in pre-carcinogenesis in hydrothermal areas, reinforcing the need for further studies with human populations living in these areas. 相似文献
69.
Variation in female sperm storage is explained, in part, by the amount of sperm transferred at mating. Laboratory mating experiments
were conducted on Eurypanopeus depressus and Rhithropanopeus harrisii from the Chesapeake Bay and Pachygrapsus transversus from Florida, while mated pairs of Uca beebei and U. terpsichores were collected from mudflats in Panama. All experiments and collections were conducted during the summer of 2006 and 2007.
More sperm was transferred to larger than smaller females, and by species with long copulation durations (R. harrisii and E. depressus). These two species live in cryptic habitats, have high sperm/egg ratios, and likely store sperm across multiple broods.
In contrast, P. transversus and U. beebei mate conspicuously, have short copulations, transfer fewer sperm, and have low sperm/egg ratios. Comparisons of sperm transfer
across different mating strategies and habitats provide a better understanding of female sperm storage in the Brachyura. 相似文献
70.
Priscila de Morais Lima Paula Loureiro Paulo 《Journal of Material Cycles and Waste Management》2018,20(3):1583-1593
Although there has been a worldwide growing concern regarding solid-waste management, the rural area has been left behind on this matter. It is common knowledge that the lack of waste management is very detrimental to the environment and impact seriously the public health. Therefore, the aim of this work is to contribute to the decision-making process regarding waste management in the rural area by proposing a new methodology using the combination of risk analysis and complementary sustainability criteria. The proposed methodology was demonstrated through a case study in the State of Mato Grosso do Sul, Brazil. A field survey was performed to define the current scenarios and three new scenarios were proposed. The risk analysis showed that the major risk to which residents are vulnerable to is the appearance of vectors that can cause various types of diseases. Besides that, only 30% of rural Quilombola communities of Mato Grosso do Sul have waste collection and the predominant scenario is the burning of dry waste and use of organic waste for animal feeding. Using the complementary relevant criteria, the most suitable scenario do the studied communities would be the combination of household composting and source separation of dry waste. The proposed methodology can be easily applied to other rural regions seeking for sustainable waste management systems. 相似文献